Simonet et al.'s*
FTW Medium
| Component | Per liter |
| Glucose | 10 g |
| Casein Hydrolyzate | 4 g |
| Yeast Extract | 0.050 g |
| Tween 80 | 2ml |
| K2HPO4 | 0.5 g |
| MgSO4.7H20 | 0.200 g |
| CaCl2.2H2O | 0.003 g |
| Citric Acid | 0.010 g |
| Fe.citrate | 0.010 g |
| Microelements | 1.0 ml |
| Vitamin Stock | 1.0 ml |
| (adjust pH to 6.7) | |
*Simonet, P., P. Normand, A. Moiroud and M. Lalonde. 1985. Restriction enzyme digestion of Frankia plasmids. Plant and Soil 87:49-60).
Vitamin Stock:
| Component | (g/l) |
| Nicotinic Acid | 0.5 |
| Pyridoxin | 0.5 |
| Thiamin | 0.2 |
Microelements:
| Component | (g/l) |
| H3BO4 | 1.5 |
| MnCl2.4H2O | 0.7 |
| ZnSO4.7H2O | 0.6 |
| CuSO4.5H2O | 0.1 |
| (NH4)6Mo7O24 | 0.020 |
| CoCl2.6H2O | 0.001 |